Poster Presentation 22nd Annual Lorne Proteomics Symposium 2017

The future of glycan MALDI mass spectrometry imaging (#108)

Matthew T. Briggs 1 , Arun Dass 2 , Yin Ying Ho 1 , Nicolle Packer 2 , Peter Hoffmann 1
  1. The University of Adelaide, Adelaide, SA, Australia
  2. Faculty of Science, Biomolecular Frontiers Research Centre, Macquarie University, Sydney, NSW

MALDI mass spectrometry imaging of N-glycans was established 4 years ago and is now routinely used by 3 groups around the World. Since our group’s proof-of-principle paper in 2015, we have published several other papers that spatially investigate the N-glycome of formalin-fixed paraffin-embedded (FFPE) tissues that have been extracted from osteoarthritis and ovarian cancer patients. However, further work is required by our group to (i) improve and optimize this workflow, and (ii) verify whether this technique can be used to discover potential diagnostic markers for early-stage diseases. This presentation will provide (i) detailed instructions on our established method for groups new to MALDI mass spectrometry imaging and (ii) a vision for the future of this new glycomic technology.   

 

 

 

  1. Ove J R Gustafsson, Matthew T Briggs, Mark R Condina, Lyron J Winderbaum, Matthias Pelzing, Shaun R McColl, Arun V Everest-Dass, Nicolle H Packer, Peter Hoffmann: MALDI imaging mass spectrometry of N-linked glycans on formalin-fixed paraffin-embedded murine kidney. Analytical and Bioanalytical Chemistry 12/2014; 407(8). DOI:10.1007/s00216-014-8293-7
  2. M Briggs, A Everest-Dass, M Oehler, N Packer, P Hoffmann: The Glycomic Quest for the Holy Grail: An Early Stage Biomarker for Ovarian.
  3. Matthew T. Briggs, Julia S. Kuliwaba, Dzenita Muratovic, Arun V. Everest-Dass, Nicolle H. Packer, David M. Findlay, Peter Hoffmann: MALDI mass spectrometry imaging of N- glycans on tibial cartilage and subchondral bone proteins in knee osteoarthritis. Proteomics 03/2016; 16(11). DOI:10.1002/pmic.201500461
  4. Arun V. Everest-Dass, Matthew T. Briggs, Gurjeet Kaur, Martin K. Oehler, Peter Hoffmann, Nicolle H Packer: N -Glycan MALDI Imaging Mass Spectrometry on Formalin-Fixed Paraffin-Embedded Tissue Enables the Delineation of Ovarian Cancer Tissues. Molecular & Cellular Proteomics 07/2016; DOI:10.1074/mcp.M116.059816