Muscle injury is a prevalent cause of debilitation for workers, athletes, and the public generally at home and in motor vehicle accidents. These incidents cause suffering to patients and even permanent disability. To date, a number of physical trauma models have been established, including in vivo which lay a foundation of research into skeletal muscle injury. While the general processes associated with skeletal muscle injury have been described.
In spite of these investigations into skeletal muscle injury, it is still unclear as to the specific mechanisms of injury progression and factors affecting the initiation of the recovery process. It is therefore necessary to conduct research aimed at providing more detailed insights into the fundamental factors and mechanisms that regulate the activity of skeletal muscle cells following injury. This may further enable the development of potential novel treatment approaches for improving the recovery from serious muscle trauma.
Methodology
The project will combine global/profiling and functional proteomics based approaches in in vivo model. Specifically, a rat impact contusion model will be utilised to model impact trauma. Dynamic global protein profiling using, LC-MS/MS will be performed at 6h, 12h, 1, 3, 7 and 14 days on tissue homogenates to identify factors that are associated with the initial recovery response following injury.
Expected Results